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Mountain Technique

Mounting is necessary to properly position an object for clear view. It is the last step in the process of slide preparation. For mounting, put a drop of suitable mounting medium such as water or glycerine or canada balsam on the center of a clean glass slide. Place the material on the mounting material with the help of a fine brush or forceps and finally place a clean cover slip on it. To place the cover slip properly, hold the cover slip with the forefinger and the thumb of the left hand and support it by a needle hold in the right hand. Lower down the cover slip slowly and touch one edge of the cover slip obliquely on one side of the mounting liquid. Holding the other edge of the cover slip with a needle point lower it down slowly to avoid air bubbles till it completely touches the slide. Suck the excess fluid outside the cover slip with the blotting paper.
The slide which is without any air bubble is considered as the good slide. Therefore, try to avoid the entrance of any air bubble.

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Posted by Unknown on 2:09 AM. Filed under . You can follow any responses to this entry through the RSS 2.0. Feel free to leave a response

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